Regulatory

Part:BBa_J100244:Experience

Designed by: Sophia Mills   Group: Campbell M Lab   (2016-01-28)


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Reactivity of sulA Promotor to Resveratrol.jpg===Applications of BBa_J100244===

We utilized the sulA promoter in an attempt to turn bacterial E.coli cells red. This promoter, according to articles researched online, was inducible by resveratrol, a reactive oxygen solution. What we found was that the promoter was leaky, so that after transformed bacterial cells with the new promoter were grown in a petri dish many of the cells which were previously green had turned red. Afterwards, we added resveratrol to separate vials of bacteria under various conditions. The positive control condition contained a strong promoter that when transcribed produce RFP. The negative control contained a solution of water and bacteria without the new promoter inserted. Two vials contained a solution of transformed bacteria, nutrients, and the resveratrol inducer. The last vial contained transformed bacteria without the presence of resveratrol. What the results indicated was that what we thought would induce the sulA promoter actually inhibited it. The resveratrol resulted in cell death as opposed to the transcription of RFP, while the vial containing sulA without resveratrol produced more RFP. The positive control, as expected, produced a large amount of RFP due to the strength of the promoter. The negative control, however, was subjected to human error. Although the bacteria inserted into this vial did not contain the sulA promoter, more RFP appeared in this vial than the two manipulated vials. This may have been due to contaminated swabs transferred between vials. When comparing the fluorescence of the vials containing the promoter and sulA to the vial only containing the promoter, there was a P value of 8.52351E-06. Thus, the difference in results between these two vials is not likely to be due to random chance.

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